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21 September, 14:08

This question deals with dilutions. It has four parts.

A) If the dilution of a sample is 1/8, what is the dilution factor (D. F.) ? I got my answer to be 8.

B) Suppose you want to make a 1/18 dilution of a sample.

If you use 7 ml of the sample, how many ml of diluent do you need to use?

(NOTE: You do not need to put units in your answer, the units in this problem are milliliters.) I got my answer to be 119.

C) In a three step serial dilution:

the first dilution is 1/6

the second dilution is 1/2

the third dilution is 1/10

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Answers (1)
  1. 21 September, 15:36
    0
    A) As the formula for Dilution factor is D. F=1/dil so D. F=1:1/8 ⇒ 8. Your answer is absolutely right.

    B) Stock solution = 7ml

    D. F=1:1/18⇒18

    Also, D. F = Final solution volume/Volume from stock solution, So:

    Final solution volume = Volume from stock solution * Dilution factor⇒7*18=126 ml

    For amount of diluent = Total solution - stock solution⇒ 126-7=119.

    119 ml of diluent is required to make 1/18 dilution of 7 ml stock solution.

    C) If we want to know the final Dilution Factor, we will simply multiply all dilutions.

    Final dilution factor will be : 1/6 x 1/2 x 1/10 = 1 : 120 or D. F = 120
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