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24 February, 23:33

A recombinant plasmid created during a recombinant DNA experiment:

A. consists only of extra-chromosomal bacterial DNA.

B. carries and allows expression of a foreign gene of interest.

C. is used in whole organism cloning.

D. can be used to insert bacterial genes into a human.

E. functions to remove specific genes from a plant.

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  1. 25 February, 01:27
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    B. carries and allows expression of a foreign gene of interest.

    Explanation:

    Plasmids are the low molecular weight extrachromosomal DNA molecules that are able to replicate themselves independent of chromosomal DNA due to the presence of "ori" (origin of replication).

    Plasmids serve as vectors in recombinant DNA technology as some of their genes can be replaced with the gene of interest to create recombinant plasmids.

    The restriction enzymes that create the sticky ends and DNA ligases facilitate the insertion of the foreign gene into the plasmids.

    The host cells are made competent to take up the recombinant plasmids. Once inside the host cell, the recombinant plasmids replicate themselves and express the gene of interest that they carry.
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