A protein retained on an ion exchange chromatography column is usually eluted off the column by (circle the correct answer)

A. increasing the salt concentration in the buffer.

B. adding the protein's free ligand.

C. changing the temperature of the elution buffer.

D. allowing the retained protein to naturally come off the column after the non-specifically bound proteins have first passed through the resin.

Question

Biology

Rayne Calhoun

2 June, 16:13

A protein retained on an ion exchange chromatography column is usually eluted off the column by (circle the correct answer)

A. increasing the salt concentration in the buffer.

B. adding the protein's free ligand.

C. changing the temperature of the elution buffer.

D. allowing the retained protein to naturally come off the column after the non-specifically bound proteins have first passed through the resin.

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Answers (1)

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Essence Jimenez · Answer 1

Option A

Explanation:

The increase in concentration of salt provides increase in ionic strength that would break the interaction of protein with chromatography matrices.