Affinity chromatography is a method that can be used to purify cell-surface receptors, while they retain their hormone-binding ability. A ligand (hormone) for a receptor of interest is chemically linked to polystyrene beads. A solubilized preparation of membrane proteins is passed over a column containing these beads. Only the receptor binds to the beads. This method of affinity chromatography would be expected to collect which of the following? A. Molecules of the hormone. B. Molecules of purified receptor. C. G protein. D. Assorted membrane proteins. E. Hormone-receptor complexes.

Question

Biology

Patterson

3 August, 18:24

Affinity chromatography is a method that can be used to purify cell-surface receptors, while they retain their hormone-binding ability. A ligand (hormone) for a receptor of interest is chemically linked to polystyrene beads. A solubilized preparation of membrane proteins is passed over a column containing these beads. Only the receptor binds to the beads. This method of affinity chromatography would be expected to collect which of the following? A. Molecules of the hormone. B. Molecules of purified receptor. C. G protein. D. Assorted membrane proteins. E. Hormone-receptor complexes.

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Brennan · Answer 1

B

Explanation:

Affinity chromatography is a purification technique. It separates/purifies proteins by exploiting their specificity or affinity to the ligand. This technique is primarily used biological molecules.