2. The mRNA for a well-known secretory protein is isolated and placed in a test tube in the presence of all the substances required for in vitro protein synthesis. The sequence of the protein produced in vitro is then compared to the sequence of the secretory protein purified from the extracellular fluid. The sequences of the two proteins are not the same. What do you predict is the difference between the two proteins and why do you see this difference

Post-transnational adjustment is a significant character of secretory protein which happens during translocation of protein through the endoplasmic reticulum and Golgi bodies. These proteins have some sign arrangement for this translocation and these successions are cut in the wake of moving toward the particular area of the cell.

If there should arise an occurrence of in vitro amalgamation of the above protein, the protein basically interpreted from the RNA and no cutting of the sign grouping and another succession which are cut in any case during translocation happens. Because of this succession of protein varies from the in vivo protein.