A microbiologist is working with two genera of bacteria, A and B, to determine if cells are competent. Strains are grown under optimal conditions, and DNA fragments that carry the gene for green fluorescent protein is added to each culture at 20-minute intervals. Samples from each treatment are plated to nutrient agar and incubated, after which the plates are examined under an ultraviolet lamp. The microbiologist observes that all plates of bacteria B contain colonies that are fluorescent, while for bacteria A only the plates from stationary phase demonstrate fluorescent colonies. What can be concluded from these results?

Question

Biology

Jaydin Kaiser

4 October, 13:29

A microbiologist is working with two genera of bacteria, A and B, to determine if cells are competent. Strains are grown under optimal conditions, and DNA fragments that carry the gene for green fluorescent protein is added to each culture at 20-minute intervals. Samples from each treatment are plated to nutrient agar and incubated, after which the plates are examined under an ultraviolet lamp. The microbiologist observes that all plates of bacteria B contain colonies that are fluorescent, while for bacteria A only the plates from stationary phase demonstrate fluorescent colonies. What can be concluded from these results?

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Lilly Klein · Answer 1

it would be an observation

Observation in scientific process refers to gathering facts and information. In this particular context, we could see that the microbiologist is simply stating what he/she saw on the mold on the agar plate. The microbiologist simply writing down all the facts without making any explanation or assumption.