Describe how to prepare 10. mL of a 5, 10, 15, and 20 uM Cv sokution using a 25 uM CV stock solution

The equation for dilutions is (concentration1) (volume1) = (concentration2) (volume 2)

For each of these, concentration 1 = 25 uM, volume 2 = 10 ml, contentration 3 is those listed. Volume 1 will be the amount of the original stock solution needed. The rest of the 10 ml will be made up with water.

5 uM = (25 uM) (V1) = (10) (5) - - > v1 = 50/25 = 2

V1 = 2 ml stock solution + (10-2) = 8ml water

10 uM = (25 uM) (V1) = (10) (10)

= 4 ml stock solution + 6 ml water

15 uM = (25) (V1) = (15) (10)

= 6 ml stock solution + 4 ml water

20 uM = (25) (V1) = (20) (10)

= 8 ml stock solution + 2 ml water