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31 January, 02:05

1. What percent of light passes through the sample if its absorbance A=2?

2. For the best calibration curve, the transmittance values (%T) should fall within the range from 10% to 90%. What is the approximate range of corresponding absorbance values?

3. What is the absorbance of the solution if the percent transmittance for it is 10%?

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  1. 31 January, 05:21
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    1. The formula for absorbance is given as:

    A = log (Io / I)

    where A is absorbance, Io is initial intensity, and I is final light intensity

    log (Io / I) = A

    log (Io / I) = 2

    Io / I = 100

    Taking the reverse which is I / Io:

    I / Io = 1 / 100

    I / Io = 0.01

    Therefore this means that only 0.01 fraction of light or 1% passes through the sample.

    2. What is meant by transmittance values is actually the value of I / Io. So calculating for A:

    at 10% transmittance = 0.10

    A = log (Io / I)

    A = log (1 / 0.10)

    A = 1

    at 90% transmittance = 0.90

    A = log (Io / I)

    A = log (1 / 0.90)

    A = 0.046

    So the absorbance should be from 0.046 to 1

    3. at 10% transmittance = 0.10

    A = log (Io / I)

    A = log (1 / 0.10)

    A = 1
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