Which ligand binds the tightest? Ligand A, with a Kd of 10^-9M Ligand B, with a Kd of 10^-3M Ligand C, with a percent occupancy of 30% at one micromolar

Ligand D, with a percent occupancy of 80% at 10 nm

Question

Chemistry

Hugo Burns

20 February, 23:47

Which ligand binds the tightest? Ligand A, with a Kd of 10^-9M Ligand B, with a Kd of 10^-3M Ligand C, with a percent occupancy of 30% at one micromolar

Ligand D, with a percent occupancy of 80% at 10 nm

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Answers (1)

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Elisa Trevino · Answer 1

To answer this item, we must take note that the ligand that binds the tightest is the one with the lowest dissociation constant, Kd. Kd's for both A and B are already given so, we only need to solve Kds for C and D.

Kd of C

0.3 = (1x10⁻⁶) / (1x10⁻⁶ + Kd); Kd = 2.3x10⁻⁶

Kd of D

0.8 = (1x10⁻⁹) / (1x10⁻⁹ + Kd); Kd = 2.5x10⁻10

Since Ligand D has the least value of dissociation constant then, it can be concluded that it binds the tightest.

Which ligand binds the tightest? Ligand A, with a Kd of 10^-9M Ligand B, with a Kd of 10^-3M Ligand C, with a percent occupancy of 30% at one micromolarLigand D, with a percent occupancy of 80% at 10 nm

Which ligand binds the tightest? Ligand A, with a Kd of 10^-9M Ligand B, with a Kd of 10^-3M Ligand C, with a percent occupancy of 30% at one micromolar

Ligand D, with a percent occupancy of 80% at 10 nm