You decide to clone a gene using a plasmid vector featuring ampicillin resistance and lacz. however, when you try to grow the

e. coli onto a plate containing x-gal and ampicillin, you see no colonies. explain what most likely went wrong during the cloning process.

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Rashad Ford

11 October, 15:01

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Agustin Molina · Answer 1

You did not do this experiment in aseptic conditions

You decide to clone a gene using a plasmid vector featuring ampicillin resistance and lacz. however, when you try to grow thee. coli onto a plate containing x-gal and ampicillin, you see no colonies. explain what most likely went wrong during the cloning process.