To clone the entire coding region of p53 into the plasmid, which restriction enzyme (s) could you use to cut both your pcr product and the pcdna3 plasmid?

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Biology

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11 July, 06:35

To clone the entire coding region of p53 into the plasmid, which restriction enzyme (s) could you use to cut both your pcr product and the pcdna3 plasmid?

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Meadow Dunlap · Answer 1

They protect bacterial cells from invasion by foreign DNA. Plasmid vector you want to use to clone a gene. The diagram shows the location of the recognition sites for four restrictions enzymes, BamHI. As the bacteria divide they will lose the plasmid. If a plasmid lacks a roots of replication.