Taq polymerase is an enzyme isolated from the organism thermus aquaticus. this organism has been found living in the hot springs of yellowstone national park. this enzyme is used to copy human dna from crime scenes. most reactions are performed at ranges similar to those of the human body; however, what considerations should be made for optimum use of this enzyme?

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Biology

Valeria Dalton

13 April, 15:04

Taq polymerase is an enzyme isolated from the organism thermus aquaticus. this organism has been found living in the hot springs of yellowstone national park. this enzyme is used to copy human dna from crime scenes. most reactions are performed at ranges similar to those of the human body; however, what considerations should be made for optimum use of this enzyme?

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Darius · Answer 1

Because Taq polymerase came from a thermophilic bacterium known as Thermus aquaticus found in Yellowstone National park, during PCR they should be kept at a specific temperature of 72C for maximum DNA strand replication and range of 75C-80C for optimum DNA strand replication.