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23 December, 13:46

Describe qualitatively how the tm for a double-stranded dna depends on its nucleotide composition

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  1. 23 December, 15:31
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    I think the higher the proportion of Guanine and cytosine; then the higher the melting temperature, tm. This is because there are three hydrogen bonds between the bases pairing between G and C, thus more thermal energy will be required to break the three hydrogen bonds holding G and C pairs, as compared to the breaking the two hydrogen bonds bonds holding A=T pairs.
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