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29 December, 13:49

Using solid-phase synthesis, you want to synthesize a 70-mer oligonucleotide with an overall yield of at least 60%. Your gene machine has a coupling efficiency of 99%. Will you be able to achieve your goal? Show the math.

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  1. 29 December, 14:00
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    No, The goal cannot be achieved

    Explanation:

    In calculating solid phase synthesis,

    Yield (Y) = CE^n-1

    CE is coupling efficiency

    n is length of oligonucleotide

    Therefore, according to the question

    CE is 99% = 0.99 (99/100)

    n is 70

    Y = 0.99^70-1 = 0.99^69 = 0.499

    Converting to percentage

    0.499 x 100=49.9% approximately 50%.
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