Biology

Mckee

9 December, 14:00

You purified fragments of nuclear envelope from human fibroblasts by biochemical fractionation. You first decide to use electron microscopy to test for the presence of the nuclear lamina on these envelope fragments. Which of the following structures would suggest the presence of the nuclear lamina?

A. "Little bumps", presumably membrane proteins, at the surface of the inner membrane

B. Two phospholipids bilayers ~300 and ~700 nm fibers An organized meshwork of ~ 10 nm fibers

C. Large pores that span through both nuclear membranes