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25 January, 19:51

There are two promoters that control synthesis of RNAs that act to prime DNA synthesis or act to inhibit that priming event. If you wanted to increase the copy number of the plasmid from 20/cell to 120/cell, what would be your strategy?

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  1. 25 January, 21:58
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    Substitute the P-II promoter (makes RNA II) for one that is stronger, or mutate the P-I promoter to make it weaker.
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