A student wishes to target hexokinase, a cytosolic enzyme of glycolysis, to the mitochondria of cells in culture as a project for a senior honor's thesis in her college. What experiment would be necessary to accomplish this task?

A) The student would need to clone the codons for a stop-transfer sequence into the gene for hexokinase in these cells.

B) The student would need to mutate and cause a defect in the gene for the signal recognition particle in these cells.

C) The student would need to clone a transit sequence into the gene for hexokinase into these cells.

D) The student would need to clone the codons for a start-transfer sequence into the gene for hexokinase in these cells

Question

Biology

Daniella Bentley

20 September, 07:19

A student wishes to target hexokinase, a cytosolic enzyme of glycolysis, to the mitochondria of cells in culture as a project for a senior honor's thesis in her college. What experiment would be necessary to accomplish this task?

A) The student would need to clone the codons for a stop-transfer sequence into the gene for hexokinase in these cells.

B) The student would need to mutate and cause a defect in the gene for the signal recognition particle in these cells.

C) The student would need to clone a transit sequence into the gene for hexokinase into these cells.

D) The student would need to clone the codons for a start-transfer sequence into the gene for hexokinase in these cells

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Answers (1)

Know the Answer?

Elle · Answer 1

Hexokinase is the first enzyme that participates in glycolysis.

Explanation:

If we want to transfer the hexokinase enzyme to the mitochondria of the cells, it is required that the transit sequence should be cloned into the gene for enzyme hexokinase into the cells. The transit sequence also known as signal sequence targets the location of the enzymes. This transit sequence is present in the N-terminal region of most of the enzyme or protein.

Answer is c.