Using current DNA-sequencing technology, it is possible to sequence an entire chromosome (e. g., human chromosome 1, which contains approximately 250 million nucleotides) as one long molecule. a) trueb) false

Question

Biology

Ashleigh Hubbard

27 August, 10:16

Using current DNA-sequencing technology, it is possible to sequence an entire chromosome (e. g., human chromosome 1, which contains approximately 250 million nucleotides) as one long molecule. a) trueb) false

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Answers (1)

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Zackary Willis · Answer 1

a) false

Explanation:

It is possible to sequence the entire genome, but not as one long molecule. By using the Massive parallel signature sequencing (MPSS), the latest technology available until now, the DNA is then fragmented into small pieces in order to be sequenced. In this technique, que marked sequences are obtained from several copies from cDNA and binded in long chains in order to create concatemers, which will be then sequenced.