Which statement regarding the Polymerase Chain Reaction (PCR) is TRUE? Group of answer choices The primers that are used to start a PCR reaction must be double-stranded and cannot be complementary to the target DNA. PCR uses a DNA polymerase that functions best at cold temperatures. A single PCR reaction can be used to duplicate an entire genome. The amount of target DNA segments doubles with each cycle of PCR.

Question

Biology

Holland

14 June, 06:48

Which statement regarding the Polymerase Chain Reaction (PCR) is TRUE? Group of answer choices The primers that are used to start a PCR reaction must be double-stranded and cannot be complementary to the target DNA. PCR uses a DNA polymerase that functions best at cold temperatures. A single PCR reaction can be used to duplicate an entire genome. The amount of target DNA segments doubles with each cycle of PCR.

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Answers (1)

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Regina Underwood · Answer 1

The amount of target DNA segments doubles with each cycle of PCR.

Explanation:

A polymerase chain reaction is an in vitro technique that is performed in the labs to replicate the DNA from a very small amount of DNA sample. The DNA sample is replicated or double in a very small amount of time that is the target sample doubles after each cycle.

The PCR technique is based on the DNA replication mechanism and thus employs the primer, A Taq DNA polymerase enzyme and the adequate amount of temperature.

Thus, the selected option is correct.