When performing column chromatography to purify a protein of interest, one needs an assay to identify which fractions that contain the protein of interest. True or False: As discussed in lecture, we use a different assay for each different protein that we purify (For instance, the assay for protein A will be different than the assay for protein B which will be different than the assay for protein C, etc).

Question

Biology

Kenyon

17 July, 13:36

When performing column chromatography to purify a protein of interest, one needs an assay to identify which fractions that contain the protein of interest. True or False: As discussed in lecture, we use a different assay for each different protein that we purify (For instance, the assay for protein A will be different than the assay for protein B which will be different than the assay for protein C, etc).

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Christine Simpson · Answer 1

True

Explanation:

Protein purification involves isolating them from their source based on differences in their physical properties. The objective in chromatography is to retain the largest amount of functional protein with the least number of contaminants, which is why an assay needs to be performed for each type of protein.