Nucleic acid has been isolated from a urethral swab and a PCR is performed on the sample in order to detect Neisseria gonorrhoeae. The positive control has a band at the expected size; the negative template control and the contamination controls do not have bands; and the amplification control band and does not have a band corresponding to the positive control band. How is this result interpreted?

Question

Biology

Macie Benitez

9 November, 16:56

Nucleic acid has been isolated from a urethral swab and a PCR is performed on the sample in order to detect Neisseria gonorrhoeae. The positive control has a band at the expected size; the negative template control and the contamination controls do not have bands; and the amplification control band and does not have a band corresponding to the positive control band. How is this result interpreted?

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Marlie Valdez · Answer 1

PCR (polymerase chain reaction) may be defined as the series of reaction used to increase the DNA product. Three main steps of PCR are denaturation, annealing and extension.

The DNA can be used to diagnose the particular disease. As the information in the question about the DNA bands of the patient. It can be concluded that the patient is not suffering from any infection and the test reveals the true negative result.