In a cell-free protein synthesis system utilizing microsomes from fragmented ER, under which condition could you determine if the new protein was imported into the microsome?

Question

Biology

Wyatt Gay

20 May, 12:38

In a cell-free protein synthesis system utilizing microsomes from fragmented ER, under which condition could you determine if the new protein was imported into the microsome?

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Answers (1)

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Jennifer Fitzpatrick · Answer 1

The correct answer is - Ribosomes and mRNA are incubated with microsomes then a protease and detergent are added and results are analyzed.

Explanation:

Microsomes are heterogenous vesicle like structures derived from fragmented endoplasmic reticulum in a cell free protein synthesis. These are formed during the process called tissue homogenization.

To find out the that the new protein was imported into the microsome by incubating the microsome with the mRNA and ribosome and then a protease and detergent to the product of incubating matter and analyze the results.

Thus, the correct answer is - Ribosomes and mRNA are incubated with microsomes then a protease and detergent are added and results are analyzed.