Which of the following places the steps in the PCR procedure in the correct order?

1) Incubate at 94°C to denature DNA strands;

2) Incubate at 72°C for DNA synthesis;

3) Incubate at 60°C for primer hybridization.

Question

Biology

Karsyn Cervantes

5 October, 18:29

Which of the following places the steps in the PCR procedure in the correct order?

1) Incubate at 94°C to denature DNA strands;

2) Incubate at 72°C for DNA synthesis;

3) Incubate at 60°C for primer hybridization.

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Answers (1)

Know the Answer?

Brody Dean · Answer 1

Polymerase chain reaction (PCR) test is used to duplicate traces of DNA from any living form, for identification purposes. The correct sequence is 1, 3, 2; based on the options given.

Explanation:

The steps to be followed for the PCR procedure are:

Denaturation

Option 1. Involves incubation at 94°C to denature DNA strands into single strands by breaking of weak hydrogen links

Annealing

Option 3. Temperature is lowered to 60°C to allow primers joining their similar DNA sequences.

Elongation

Option 2. Incubate at 72°C to promote the extension of the DNA strands, actually extending them.

Which of the following places the steps in the PCR procedure in the correct order?1) Incubate at 94°C to denature DNA strands;2) Incubate at 72°C for DNA synthesis;3) Incubate at 60°C for primer hybridization.

Which of the following places the steps in the PCR procedure in the correct order?

1) Incubate at 94°C to denature DNA strands;

2) Incubate at 72°C for DNA synthesis;

3) Incubate at 60°C for primer hybridization.