2. How are the site (s) where replication begin (s) is/are characterized as in both prokaryotes and eukaryotes

Answer: In prokaryotes, site for replication origin is a short stretch of nucleotides which contains multiple binding site. Prokaryotes have single origin of replication. While eukaryotes have multiple origin of replication sites.

Origin of replication has the specific bases pairs that are complementary to replication initiation protein which will find to it.