Refer to the Biochemistry in Focus section of your text for this chapter to answer this question. Instead of using acidic conditions, how could the research team release chymotrypsin from the column? A) Treat the column with additional chymotrypsin. B) Treat the column with a concentrate solution of glucose. C) Treat the column with a concentrated solution of tryptophan. D) Treat the column with a concentrated solution of proline.

D) Treat the column with a concentrated solution of proline.

Explanation:

Instead of using acidic conditions, the research team can enhance the release of chemotrypsin from the columns by treating the column with a concentrated solution of proline.

Proline is an imino acid not an amino acid. Nonetheless it is called an amino acid and it is involved in the biosynthesis of protiens. Proline can also regulate development and bio0synthesis of proiline is important for the maintenence of NADP + pools during stress.

Hence D) Treat the column with a concentrated solution of proline. is the right option.

Instead of using acidic conditions, how could the research team release chymotrypsin from the column, we can have the following treatment for the process to be executed properly.

Explanation:

A) Treat the column with additional chymotrypsin.

B) Treat the column with a concentrate solution of glucose.

C) Treat the column with a concentrated solution of tryptophan