During purification of many cytosolic proteins, it is important to

include either dithiothreitol or b-mercaptoethanol in the

buffers. Why?

Question

Biology

Jillian Petty

10 July, 01:54

During purification of many cytosolic proteins, it is important to

include either dithiothreitol or b-mercaptoethanol in the

buffers. Why?

+1

Answers (1)

Know the Answer?

Michaela Kaiser · Answer 1

To reduce thiol-groups of the protein

Explanation:

Dithiothreitol of b-mercaptoethanol act as reducing agents. Including them in a buffer would prevent oxidation of thiol-groups in amino acids of the protein and preventing its denaturation.

During purification of many cytosolic proteins, it is important toinclude either dithiothreitol or b-mercaptoethanol in thebuffers. Why?

During purification of many cytosolic proteins, it is important to

include either dithiothreitol or b-mercaptoethanol in the

buffers. Why?