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23 January, 18:05

Primase is an enzyme that synthesizes RNA polymers to provide a 3'OH end for DNA polymerase to use. Which of the following is Primase going to need?

a. a DNA template and deoxyribonucleoside triphosphates (dNTPs)

b. an RNA template and ribonucleoside triphosphates (NTPs)

c. a DNA template and ribonucleoside triphosphates (NTPs)

d. an RNA template and deoxyribonucleoside triphosphates (dNTPs)

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  1. 23 January, 21:54
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    c. a DNA template and ribonucleoside triphosphates (NTPs)

    Explanation:

    Primase is an enzyme that makes short RNA fragments using DNA as a template. For the purpose, the primase enzyme uses the ribonucleoside triphosphates (NTPs) and makes an RNA copy of the existing DNA template.

    The enzyme is DNA primase as it uses the DNA template strand. The newly formed short RNA fragments are called primers. Primers are required by DNA polymerases which in turn are the enzymes of elongation only and can not start the de novo synthesis of new DNA strand.

    The free 3' OH end of the RNA primer is extended by DNA polymerase by adding deoxyribonucleotides.
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