A binding protein binds to a ligand, L, with a Kd of 400 nM. How much ligand (in nM) is present when the fraction of ligand bound is 60%?

Question

Biology

Emmy Mcgrath

9 May, 03:04

A binding protein binds to a ligand, L, with a Kd of 400 nM. How much ligand (in nM) is present when the fraction of ligand bound is 60%?

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Answers (1)

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Hector Guerra · Answer 1

600nM

Explanation:

From Concentration of Ligand = Ykd/1 - Y where Kd = 400nM. fraction of ligand bound Y = 0.60

Plugging the values of Y and Kd; Concentration = 0.6 x 400 / 1 - 0.6 Concentration = 600 x 10^-9 = 600nM