Which of the proteins are directly responsible for ensuring that eukaryotic DNA is replicated precisely once in each round of replication, even though there are thousands of replication origins?

Answer: DNA polymerase

Explanation:

DNA polymerase is the enzyme protein with a nearly accurate proofreading ability to identify the DNA strand meant for replication.

Among thousands of replication origins, the DNA polymerase identifies the promoters to be used, thus ensuring accuracy in DNA replication