Researchers often want to isolate a certain type of RNA. For some RNA species, this can be accomplished via affinity chromatography, using beads coated with chains of poly-deoxythymidine (poly-dT). The desired RNA will stick to the beads while unwanted RNAs will flow through the column. The retained RNA can then be eluted. What RNA species can be purified using this method?

Question

Biology

Speedo

2 June, 03:58

Researchers often want to isolate a certain type of RNA. For some RNA species, this can be accomplished via affinity chromatography, using beads coated with chains of poly-deoxythymidine (poly-dT). The desired RNA will stick to the beads while unwanted RNAs will flow through the column. The retained RNA can then be eluted. What RNA species can be purified using this method?

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Answers (1)

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Antony Donovan · Answer 1

mRNA

Explanation:

poly-deoxythymidine (poly-dT) beads binds to polyadenylated (poly (A) molecules with high affinity because Thymidine base pairs with the Adenine of the poly (A) chain.

Since messenger RNA (mRNA) is polyadenylated after transcription in contrast to other RNA species, this method is more suitable for mRNA purification.