According to the Lab Safety Sheet provided for this week, which of the following is a potential hazard you will face in the b-galactosidase assay lab? (Check all that apply) Select one or more:

a. Water reactive chemicals

b. Corrosive chemicals

c. Chemicals causing respiratory tract, eye and skin irritation

d. Potentially carcinogenic chemicals

e. Radioactive materials

Question

Biology

Ryleigh Gay

9 September, 20:07

According to the Lab Safety Sheet provided for this week, which of the following is a potential hazard you will face in the b-galactosidase assay lab? (Check all that apply) Select one or more:

a. Water reactive chemicals

b. Corrosive chemicals

c. Chemicals causing respiratory tract, eye and skin irritation

d. Potentially carcinogenic chemicals

e. Radioactive materials

+3

Answers (1)

Know the Answer?

Kenley Barrera · Answer 1

Chemicals causing respiratory tract, eye and skin irritation.

Potential Carcinogenic chemicals

Explanation:

b-galactosidase is an enzyme encoded by Lac Z gene of the lac operon in E. coli. It hydrolyses b-galactosides and lactose into monosaccharides which are simple sugars

In order to carry out this assay, materials including b-mercaptoethanol and ONPG (o-nitrophenyl-b-d-galactopyranoside) are used.

b-Mercaptoethanol produces vapors which can cause respiratory tract irritation if inhaled and ONPG is a potential carcinogenic chemical.