What determines the function of a protein apex?

The function of a protein determined by:

The shape determines the working of a protein. A protein's design is determined by its primary structure i. e the amino acid sequence. The amino acid sequence within a protein is decided by the encoding arrangement of nucleotide in the gene (DNA).

The role of a protein is utterly dependent on its three-dimensional architecture (other than when it caters as food). This framework may be disturbed by a number of agents thereby denaturing the protein:

Variations in the pH (between charged amino acids the electrostatic interactions is changed). difference in concentration of salt (does the same). temperature change (higher temperatures decrease the strength of hydrogen bonds). reduction agent presence (disruption of S-S bonds among cysteines).