George Palade's research group used the pulse-chase assay to dissect the secretory pathway in pancreatic cells. If they had instead performed this assay on muscle cells, which have high energy demands and primarily consist of actin and myosin filaments, where would you expect the labeled proteins to go during the chase?

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Biology

Clayton Pineda

23 October, 23:11

George Palade's research group used the pulse-chase assay to dissect the secretory pathway in pancreatic cells. If they had instead performed this assay on muscle cells, which have high energy demands and primarily consist of actin and myosin filaments, where would you expect the labeled proteins to go during the chase?

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Terry Osborn · Answer 1

The labeled proteins would mostly be located in the mitochondria and cytosol. The cytosolic proteins usually comprise proteins such as the myosin and the actin that ensure the contraction of muscles. Due to the presence of high energy demand, it can be assumed that several proteins would definitely need to generate ATP in the mitochondria.

Explanation:

The labeled proteins would mostly be located in the mitochondria and cytosol. The cytosolic proteins usually comprise the proteins such as the myosin and the actin that ensure the contraction of muscles. Due to the presence of high energy demand, it can be assumed that several proteins would definitely need to generate ATP in the mitochondria.