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Duncan Cohen
30 November, 17:37
The vectors used to transmit a gene segment is a
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Averie Bowen
30 November, 20:13
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plasmid
Explanation:
A cloning vector is a DNA molecule that takes the target DNA to the host cell and replicates there. A cloning vector must have a sequence that allows it to replicate within a bacterial host cell. It must have a cloning site, that is, a place to insert the target DNA.
Generally this vector is a plasmid, but other structures such as phages, cosmids and yeasts can be used.
In general, the cloning procedure, regardless of the vector, includes an initial phase of treatment of the vector and the DNA under study with restriction enzymes, in order to originate complementary terminals. The next step is to join the target DNA to the vector with the DNA ligase. Then, the target DNA is introduced into the host cell by transformation. Finally, cells that contain the target DNA are selected using markers such as antibiotic resistance.
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Gregory Compton
30 November, 20:45
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plasmid
Explanation:
Plasmid vectors are used to transmit gene segments. Plasmid vectors are used as "cloning vectors" which are small genetic pieces taken from an organism's cell. These are used for maintaining stability when foreign DNA gets inserted into the cell itself.
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