What would happen to the insulin molecule if the amino acid were assembled in a different order

The correct answer would be the loss of its function.

Being protein in nature, the function of insulin depends on the amino acid sequence of the polypeptide chains.

Change in the amino acid sequence may result in a change of shape or structure of the protein.

Consequently, it would not be able to interact with cell surface receptors.

Thus, it would lose its function.