A biology graduate student presented a seminar where he described an experiment in which two closely related Drosophila species have been crossed. Both parental species and the F1 generation have been studied using RNA-seq. For reads with single nucleotide polymorphisms (SNPs) between the two species, he could determine from which allele they had been transcribed. He was interested in finding genes with differential expression levels between species caused by trans-acting mutations. Which genes should he select

Question

Biology

Kaleigh Rosales

29 November, 13:21

A biology graduate student presented a seminar where he described an experiment in which two closely related Drosophila species have been crossed. Both parental species and the F1 generation have been studied using RNA-seq. For reads with single nucleotide polymorphisms (SNPs) between the two species, he could determine from which allele they had been transcribed. He was interested in finding genes with differential expression levels between species caused by trans-acting mutations. Which genes should he select

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Karsyn Phillips · Answer 1

In this case, the biology graduate have to select species-specific genes and then identify if they were expressed in RNA-seq data

Explanation:

By RNA-seq it is possible to identify mRNAs specifically expressed between species since homologous species-specific genes may show single nucleotide polymorphisms (SNPs) among alleles with a biased gene expression