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8 October, 12:09

Outside the

box

Starch is digested by the enzyme amylase.

A student did an investigation on amylase activity.

This is the method used.

1. Mix amylase solution and starch suspension at 20 °C in a boiling tube.

2. Remove a drop of the mixture every 30 seconds and test it for starch.

3. Continue testing every 30 seconds until no starch is detected.

4. Repeat the investigation at different temperatures.

1

What apparatus should the student use to control the temperature of the mixture?

[1 mark]

+5
Answers (1)
  1. 8 October, 12:57
    0
    An incubator.

    Explanation:

    Most enzymes work best at about 37 °C and start to denature around 40 °C.

    Thus, the student should use a laboratory incubator, which is essentially an oven that can heat a biological sample to a set temperature.

    This model shown below reaches a maximum temperature of 62° C, and its heat controller maintains temperatures within ± 0.5 °C°.
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