In blue-white screening, what do blue colonies represent?

cells that have not taken up the plasmid vector

cells with recombinant plasmids containing a new insert

cells containing empty plasmid vectors

cells with a non-functional lacZ gene

Question

Biology

Tyshawn Nelson

23 November, 15:33

In blue-white screening, what do blue colonies represent?

cells that have not taken up the plasmid vector

cells with recombinant plasmids containing a new insert

cells containing empty plasmid vectors

cells with a non-functional lacZ gene

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Answers (1)

Know the Answer?

Iris Buckley · Answer 1

The correct option is : cells containing empty plasmid vectors

Explanation:

The blue-white screen is a technique which involves the rapid identification of the recombinant bacteria in a vector-based molecular cloning experiment. In this method, a DNA ligated vector is inserted in a host cell which is viable for transformation and grown in presence of X-gal.

The cells that are transformed with the vectors having the recombinant DNA produce the white colonies. Whereas, the cells transformed with empty vector i. e. the non-recombinant plasmids, produce the blue colonies.

In blue-white screening, what do blue colonies represent?cells that have not taken up the plasmid vectorcells with recombinant plasmids containing a new insertcells containing empty plasmid vectorscells with a non-functional lacZ gene

In blue-white screening, what do blue colonies represent?

cells that have not taken up the plasmid vector

cells with recombinant plasmids containing a new insert

cells containing empty plasmid vectors

cells with a non-functional lacZ gene