What are the limitations of using an agarose gel to determine if you are homozygous or heterozygous with respect to the D1S80 locus?

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Biology

Zaiden Gardner

5 December, 16:39

What are the limitations of using an agarose gel to determine if you are homozygous or heterozygous with respect to the D1S80 locus?

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Adyson Trujillo · Answer 1

The resolution of agarose gel.

Explanation:

The resolution of a common agarose gel is not high enough to be able to differentiate the genetic differences (polymorphisms) that can produce the VNTR gene, located at the D1S80 locus. The VNTR fragment produces variants between 10-50pb, which means that a highly trained eye and a very good quality agarose will be required to differentiate a homozygous from a heterozygous individual.