You discover a pattern of a 20 amino acid sequence at the C-terminus of over 50 proteins in an organism. You hypothesize that this amino acid sequence is a signal sequence for localization into a specific organelle or cell compartment, but you don't know which one. How might you use microscopy to investigate the location of proteins with this unique C-terminus sequence?

Question

Biology

Felix Rojas

12 December, 02:29

You discover a pattern of a 20 amino acid sequence at the C-terminus of over 50 proteins in an organism. You hypothesize that this amino acid sequence is a signal sequence for localization into a specific organelle or cell compartment, but you don't know which one. How might you use microscopy to investigate the location of proteins with this unique C-terminus sequence?

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Answers (1)

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Roderick Koch · Answer 1

It depends on the type of microscope that you have

Explanation:

For example, in the case that you have a fluorescence microscope, it is possible to create a fusion construct of the domain fused to the Green Fluorescence Protein (GFP) and then introduce it into the cell. The observation of fluorescence from this construct enables to confirm the existence of the signal sequence (i. e., the C-terminal domain) in the organelle