How does the use of different restriction enzymes affect the size of the DNA bands produced?

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Biology

Merle

9 May, 02:40

How does the use of different restriction enzymes affect the size of the DNA bands produced?

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Lucifer · Answer 1

Restriction enzymes are DNA-cutting enzymes. Each enzyme recognizes one or a few target sequences and cuts DNA at or near those sequences.

Many restriction enzymes make staggered cuts, producing ends with single-stranded DNA overhangs. However, some produce blunt ends.

DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA.

In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.