Explain why it is necessary to use the same restriction enzyme to cut the desired gene from the source and to cut the plasmid. Include a drawing or diagram in your answer.

Question

Biology

Alejandro Ellis

4 October, 00:58

Explain why it is necessary to use the same restriction enzyme to cut the desired gene from the source and to cut the plasmid. Include a drawing or diagram in your answer.

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Answers (1)

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Zain Glenn · Answer 1

It is necessary because of the base pairing between the desired gene and plasmid

Explanation:

Restriction enzymes are enzymes that recognize the specific DNA sequence-restriction site, double-stranded cut DNA and produce ends with a single-stranded overhang. The overhangs of the plasmid can stick together with overhangs of gene of interest by complementary base pairing. Joining of ovarhangs is done by the enzyme DNA ligase.