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15 February, 02:25

Peptides can be separated according to their charges. indicate which peptides would elute last from a cation exchange column and an anion exchange column.

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  1. 15 February, 03:30
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    Cation-exchange chromatography is used when the molecule of interest is positively charged on the neutral pH. The stationary phase is than negatively charged and positively charged molecules are loaded to be attracted to it. So, positively charged peptides will elute the last.
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