Describe a situation that illustrates why it is a good idea to complete the elisa assay in triplicate.

Assaying the samples in triplicate is a way of control.

Using triplicates will allow you to account for variation within the assay (intra-assay variation). This means that if you do not get the same result in all triplicate wells, you probably have a problem with experimental technique or you have made a pipetting error, so the experiment should have be repeated.