In bacteria, a partially diploid strain may result from Choose one or more: A. acquisition of an F' factor. B. acquisition of F factor. C. natural transformation (not electroporation). D. generalized transduction. E. specialized transduction.

Question

Biology

Marcel Nixon

4 September, 06:07

In bacteria, a partially diploid strain may result from Choose one or more: A. acquisition of an F' factor. B. acquisition of F factor. C. natural transformation (not electroporation). D. generalized transduction. E. specialized transduction.

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Fowler · Answer 1

(A). Acquisition of an F' factor.

(E). Specialized transduction.

Explanation:

Two processes to create a partial diploid in bacteria are specialized transduction (also called restricted transduction) and F' conjugation. Specialized (or Restricted) Transduction. A lysogenic bacteriophage can excise itself so as to carry a piece of host DNA by mistake. The phage will now carry a second copy of an allele (or linked alleles) into a host cell. The new bacterium is a partial diploid for the allele.

In biotechnology, a phage chromosome can have a piece of foreign DNA ligated into it in the test tube. Then the phage DNA is packaged into phage, and it can infect a new host where it either (1) produces many copies of the host gene; or (2) lysogenizes the host, to express the cloned DNA.

2. F' plasmid. The F plasmid can recombine itself into the host chromosome, then recombine itself out again with some host DNA by mistake. When it enters the next host cell, it carries a second copy of several genes; again, a partial diploid is created. In biotechnology, a plasmid can have a piece of foreign DNA ligated into it in the test tube; then the plasmid is transformed into E. coli. Then the plasmid makes many copies, including the cloned gene.