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24 May, 08:21

A slow-growing cherry-red colored mold with numerous microconidia and no macroconidia was isolated from a sample of skin scrapings. What two things would you do to identify this organism

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  1. 24 May, 11:06
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    1. Growth on cornmeal agar

    2. KOH prep

    Explanation:

    Cornmeal agar is a nutritional deficient media containing:

    Corn meal 8g / zein 40 g / 100ml Agar 4g Distilled water 200 ml Large, highly refractory, thick walled.

    KOH preparation (10 - 30%) used to destroy keratin, leaves fungal hyphae.

    Can add a chitin-specific stain Calcofluor white fluorescent stain increases sensitivity

    Hence the organism can easily be identified by these two tests.
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