The primers you used in lab amplified 145 base pairs that were adjacent, but not included in the repeat. If you used a different primer that only amplified 130 base pairs adjacent to a 14 base pair repeat, and you had 6 repeats in your product, what was the size of your final PCR product?

Question

Biology

Marley Kirk

23 March, 13:18

The primers you used in lab amplified 145 base pairs that were adjacent, but not included in the repeat. If you used a different primer that only amplified 130 base pairs adjacent to a 14 base pair repeat, and you had 6 repeats in your product, what was the size of your final PCR product?

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Answers (1)

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Amy Lowery · Answer 1

The answer is 214.

Explanation:

This is because 130 base pair is adjacent to 14 base pair repeat, and you have 6 repeats. So 14 multiplied by 6 repeats is 84. If you add this to your primer amplicon, you will get the size of final PCR product i. e ... 214.