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10 September, 03:41

In some cases, recombinant DNA must be cloned before it can be inserted into a host. Which vectors can be used to clone recombinant DNA?

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  1. 10 September, 07:00
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    I believe these are the steps

    1. Isolate DNA from the organism

    2. Cut DNA with restriction enzymes

    3. Insert DNA fragments into a "vector" (plasmid)

    4. Introduce recombinant DNA into bacterial host cell (E. coli) for replication

    5. Grow the host and reisolate recombinant DNA clones

    **to distinguish host cells carrying plasmids with dna inserts, the vector should have a selectable marker gene - usually an antibiotic gene or an enzyme absent from host cell, when plated on this antibiotic it will be obvious to see host cells.
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