Summarize three isolation techniques. explain which method is utilized most often in a laboratory and how a colony produced by this method can be used to create a pure culture

Three isolation techniques used in laboratories are the pour plate method, the spread plate method, and the streak plate method. The pour play method entails placing the inoculum in a petri dish and pouring melted agar into the dish. The spread plate method involves placing the inoculum on a prepared petri dish of agar and spreading it evenly over the agar. The streak plate is the most common method used. In it, a small sample of the inoculum is collected on a sterile loop and spread over the petri dish, leaving a decreasing amount of inoculum along the streak path so that bacteria are more easily isolated into homogenous colonies.

The three common lab isolation techniques are: quadrant streak, loop dilution and spread plate. Isolation is used for the separation of microbes from a mixed culture into isolated colonies for the purpose of creating a pure culture. The pure culture contains only one type of known microbe. The Streak Plate Method is most commonly used to isolate pure culture.