Which of the following statements regarding Anfinsen's denaturing experiments with ribonuclease A (RNAse A) are valid? Exposing the denatured protein to air oxidation and then dialysis to remove urea restored the protein to its original functionality. Removing urea by dialysis and then allowing air oxidation of the denatured protein restored the protein to its original functionality. Denaturing the protein with both urea and β-mercaptoethanol yielded an inactive protein. Anfinsen concluded that protein folding is determined by its primary sequence.

Question

Chemistry

Damon Liu

17 November, 20:52

Which of the following statements regarding Anfinsen's denaturing experiments with ribonuclease A (RNAse A) are valid? Exposing the denatured protein to air oxidation and then dialysis to remove urea restored the protein to its original functionality. Removing urea by dialysis and then allowing air oxidation of the denatured protein restored the protein to its original functionality. Denaturing the protein with both urea and β-mercaptoethanol yielded an inactive protein. Anfinsen concluded that protein folding is determined by its primary sequence.

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Yeller · Answer 1

B, C, and D

Explanation:

Anfinsen found that denaturing the protein with both urea and β-mercaptoethanol yielded an inactive protein.

Removing urea by dialysis and then allowing air oxidation of the denatured protein restored the protein to its original functionality.

He concluded that protein folding is determined by its primary sequence.

A is false. Anfinsen removed the urea first and then allowed the enzyme solution to re-oxidize slowly in air.